Effects of Teucrium Polium Aerial Parts extract on oral glucosetolerance tests and pancreas histopathology inStreptozocin-induced diabetic rats

Document Type : Original Article

Authors
Durdi Qujeq 1 , 2
Farideh Feizi 3
Hadi Parsian 1 , 2
Alieh Sohan Faraji 3
Sohrab Halalkhor 2
Roya Abassi 2
Zeinab Abedian 1
Roughayeh Pourbagher 1
Seyed Mohsen Aghajanpour Mir 1
Hamed Mir 3
Nayer Seyfizadeh 3
Mohsen Tatar 2
1 Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran
2 Department of Biochemistry & Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
3 Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Abstract
Teucrium polium can reduce serum glucose. There are few reports in the literature related to this subject and the
resolution of this mechanism requires further experiments. The aim of the present study was to evaluate the
effects of Teucrium polium aerial parts extracts on oral glucose tolerance tests and pancreas histology in
streptozocin-induced diabetic rats. In order to prepare the aqueous concentrate, aerial parts extract was dissolved
in distilled water and was boiled for 30 minutes. For the preparation of ethanolic solution, powder was dissolved
in ethanol and mixed by a shaker. Diabetic rats were induced with single IP injection of streptozotocin (STZ) at a
dose of 50 mg/kg body weight dissolved in normal saline just before use to the 16 hr fast rats. Both groups,
diabetic and normal were sacrificed by ether anesthesia. The tissue samples were formalin fixed and paraffin
embedded for microscopic examination in accordance with routine laboratory procedures. Blood was collected
from the tail vein of the rats. Serum glucose levels were then measured by commercial kits by using a glucose
oxidized method. There were no biochemical abnormalities or histological changes in the pancreas of control
rats. Post treatment of Teucrium polium aerial parts extract reduced the severity of streptozotocin diabetic
pancreases. Our histopathological investigation along with the biochemical evaluations showed a significant
effect on histological changes in the pancreas of induced diabetic rats upon Teucrium polium aerial parts extract
treatment (P<0.05).
Keywords
Teucrium polium
oral glucose tolerance tests
pancreas histology
streptozocin
diabetic rats