Evaluation of PKM2 and MAPK8IP2 polymorphism in Ameloblastic Carcinoma: A retrospective quantitative study

Document Type : Original Article

Authors
Abbas Khodayari 1
Seyyed Mohammad Hossein Ghaderian 2
Mohammad Jafarian 1
Alireza Jahangirnia 3
Alireza Nayebi 3
Fahimeh Akhlaghi 1
Nasim Taghavi 4
Reza Akbarzadeh Najar 2
Sanaz Tabarestani 2
Arash Khojasteh 1
Sarah Aghabozorg Afjeh 2
1 Department of Oral and Maxillofacial Surgery, Dental Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Department of oral and maxillofacial pathology, dental faculty, Shahid Beheshti university of medical science, Tehran,Iran
Abstract
Ameloblastic carcinoma (AC) is a rare malignant epithelial odontogenic tumor that histologically retains the
features of ameloblastic differentiation and exhibits cytological features of malignancy in the primary or
recurrent tumor. It may develop within a preexisting ameloblastoma or arise de novo or from an odontogenic
cyst. Epidemiological evidence shows that human cancer is generally caused by genotoxic factors, genes
involved in the susceptibility of cancer, including those involved in metabolism or detoxification of genotoxic
environment and those controlling DNA replication. Nowadays, gene polymorphism has an important role in
development of malignant tumor .We report a case series study of ameloblastic carcinoma and ameloblastoma to
show the role of PKM2 and MAPK8IP2 polymorphisms in these tumors. The DNA was extracted separately
from specimens in paraffin sections of the tumor. Polymorphism of these genes was determined by PCR-RFLP
(Polymerase Chain Reaction-Restriction fragment length polymorphism) method. The allele distributions of all
samples were in Hardy-Weinberg equilibrium. The genotype and allele distribution in these genes were not
statistically different between patients and controls.
Keywords
Ameloblastic carcinoma
PKM2
MAPK8IP2
polymorphism