Determination of ctxAB Expression in Vibrio cholerae Classicaland El Tor Strains using Real-Time PCR

Document Type : Original Article

Authors
Seyed Mahmoud Amin Marashi 1 , 2
Ramazan Rajabnia 3
Abbas Ali Imani Fooladi 4
Zohreh Hojati 5
Sharareh Moghim 6
Bahram Nasr Esfahani 6
1 Cellular and Molecular Biology Research Center, (CMBRC), Babol University of Medical Sciences, Babol, Iran
2 Department of Microbiology and Immunology, Babol University of Medical Sciences, Babol, Iran
3 Infectious Diseases & Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
4 Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
5 Genetics Division, Biology Department, Faculty of Sciences, University of Isfahan, Isfahan, Iran
6 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health
threat and also a major cause of death worldwide and especially in developing countries. The major virulence
factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse
transcription was performed for making ctxAB cDNA. Relative Real-Time PCR analysis showed unequal
enterotoxin production in V. cholerae strains. The results showed that, classical strain produces cholera toxin
more than El Tor strain.
Keywords
Vibrio cholerae
RT-qPCR
ctxAB expression