A comparative study of PCNA and Ki-67 expression in dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma

Document Type : Original Article

Authors
Shima Nafarzadeh 1 , 2
Maryam Seyedmajidi 1 , 2
Sina Jafari 3
Ali Bijani 4
Ali Rostami-Sarokolaei 5
1 Dental material research center, Babol University of Medical Sciences, Babol, Iran
2 Department of Oral and Maxillofacial Pathology, Dentistry school, Babol University of Medical Sciences, Babol, Iran
3 Student Research Committee, Babol University of Medical Sciences, Babol, Iran
4 Non-communicable Pediatric Diseases Research Center, Babol University of Medical Sciences, Babol, Iran
5 Dentistry school, Babol University of Medical Sciences, Babol, Iran
Abstract
Various cell proliferation markers are used as diagnostic and prognostic tools in oral lesions. Simultaneous
evaluation of these markers can increase the precision of estimation of the proliferative status of different tissues.
In this study we investigated the expression of PCNA and Ki-67 as markers of cell proliferation in 15 paraffin
embedded samples of each dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma
belonging to a total of 30 male and 30 female paients using immunohistochemistry method. Expression levels
based on the intensity and the percentage of stained cells was separately analyzed for each marker with chisquare test, the results of which were significant for the two markers (P<0.05). The correlation coefficient
between the two markers was found to be 0.88. A significant difference in the expression of Ki-67 and PCNA
was observed in the four types of studied lesions.
Keywords
PCNA
Ki-67
immunohistochemistry
dental follicle
dentigerous cyst
ameloblastoma