The Assessment of Early Glycosaminoglycan Concentration Changes in the Kidney of Diabetic Rats by Critical Electrolyte Concentration Staining

Document Type : Original Article

Authors
Mohsen Pourghasem 1
Ebrahim Nasiri 2
Shima Sum 3
Hamid Shafi 4
1 Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran
2 Department of Anatomical Sciences, GilanUniversity of Medical Sciences, Rasht, Iran
3 Department of Social Medicin, Babol University of Medical Sciences, Babol, Iran
4 Department of Urology, Babol University of Medical Sciences, Babol, Iran
Abstract
Glycosaminoglycan (GAG) has a pivot role in renal function and homeostasis. Analysis of GAG amount
generally serves to determine GAG alteration due to diabetes mellitus. Critical Electrolyte Concentration (CEC)
staining can be an efficacy method to study GAG amount changes. Based on an experimental study, 20 male rats
were randomly divided equally into two experimental and control groups. Diabetes mellitus was induced by a
single sub cutaneous injection (120 mg/kg) of alloxan monohydrate. After 8 weeks, diabetic kidneys were
paraffin embedded and sectioned at 5µm on a microtome. Slides were prepared and studied after staining by
Critical Electrolyte Concentration (CEC 1 -4). In this study, we succeeded to show a decrease of hyaluronic acid
and chondroitin sulfate concentration in diabetic kidney at 8 weeks diabetic rats which are earlier signs
compared to those reported previously. In contrary, no significant changes in heparin sulfate and keratin sulfate
have been seen. Diabetic nephropathy is a progressive disease and earlier diagnosis makes a better treatment
design to reduce its development. CEC staining is able to determine degradation of hyaluronic acid and
chondroitin sulfate synthesis in diabetic kidney of rats in an earlier time.
Keywords
Glycosaminoglycan
diabetes mellitus
kidney
CEC