p53 and PCNA Expression in Keratocystic Odontogenic Tumors Compared with Selected Odontogenic Cysts

Document Type : Original Article

Authors
Maryam Seyedmajidi 1
Shima Nafarzadeh 2
Sepideh Siadati 3
Shahryar Shafaee 4
Ali Bijani 5
Nazanin Keshmiri 6
1 Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran
2 Oral and Maxillofacial Pathology Department, Dental Faculty, Babol University of Medical Sciences, Babol, Iran
3 Pathology Department, Medical Faculty, Babol University of Medical Sciences, Babol, Iran
4 Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran
5 Non-Communicable Pediatrics Diseases Research Center, Babol University of Medical Sciences, Babol, Iran
6 Student Research Committee, Babol University of Medical Sciences, Babol, Iran
Abstract
p53 and PCNA expression in keratocystic odontogenic tumors compared with selected odontogenic cysts
Summary: The aim of this study was to evaluate p53 and PCNA expression in different odontogenic lesions
regarding their different clinical behaviors. Slices prepared from 94 paraffin-embedded tissue blocks (25
radicular cysts (RC), 23 dentigerous cysts (DC), 23 keratocystic odontogenic tumors (KCOT) and 23 calcifying
cystic odontogenic tumors (CCOT)) were stained with p53 and PCNA antibodies using immunohistochemistry
procedure. The highest level of p53 expression was in the basal layer of RC, and the highest level of PCNA
expression was in the suprabasal layer of KCOT. The differences of p53 expression in basal and suprabasal
layers as well as PCNA expression in the suprabasal layer were significant but there was no significant
difference in PCNA expression in the basal layer of these lesions. The expression of p53 in the basal layer of RC
was higher than in other cysts. This may be due to intensive inflammatory infiltration. Also, the high level of
PCNA expression in the suprabasal layer of KCOT may justify its neoplastic nature and tendency to recurrence.
KCOT and calcifying cystic odontogenic tumors did not show similar expression of studied biomarkers.
Keywords
p53
PCNA
radicular cyst
dentigerous cyst
keratocystic odontogenic tumor
calcifying cystic odontogenic tumor