Screening of Different Organs of Rats for HCA2 Receptor mRNA

Document Type : Short Communication

Authors
Tahoora Shomali 1
Najmeh Mosleh 1
Mohammad Kamalpour 2
1 Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
2 Division of Pharmacology and Toxicology, Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract
Interest in hydroxy - carboxylic acid 2 (HCA2) receptor has been raised since it is the target of antidyslipidemic
drug nicotinic acid. The present study aimed to evaluate the presence of mRNA of this receptor in different
organs of laboratory rat. Twenty two different organs of rats including mesenteric fat, epididymis (head, body
and tail), testis, ovary, xiphoid process, liver, adrenal gland, femoral head, proximal epiphyseal and metaphyseal
bone marrow of femur, esophagus, glandular stomach, forestomach, intestines, colons, heart, spleen, kidney,
trachea, lung, skeletal muscle (quadriceps), cerebrum and cerebellum were removed and examined for HCA2
mRNA by RT- PCR method. The mRNA for HCA2 receptor was detected in all analyzed tissues. In conclusion,
the different organs of rat express HCA2 receptor mRNA which makes a proper animal model for future studies
on the physiological and pharmacological roles of this receptor in vivo.
Keywords
HCA2 receptor
mRNA
rat
RT-PCR