1
Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
2
Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Our previous studies showed that various extracts of Persian shallot (Allium hirtifolium) have anti- angiogenic effects. This study has been undertaken to isolate and identify the major effective anti- angiogeneic sub-fraction of shallot. After preparation of the 50% hydroalcoholic extract of shallot bulbs, the extract was successively fractionated into n- hexane, ethyl acetate, n- butanol and aqueous fractions. Anti-angiogenesis activity of fractions was examined by in vitro angiogenesis assay. The ethyl acetate fraction which had the most anti- angiogenesis activity was further fractionated to four sub- fractions by thin layer chromatography (TLC), silica gel column chromatography and then analyzed by High Performance TLC (HPTLC) with ethyl acetate- methanol- water as the solvent system. Our results showed that one of the four sub- fractions, as the major band in HPTLC, had the most anti- angiogenic activity. Purification and characterization of the major anti- angiogenic compound/compounds of shallot's extract may constitute one means by which diets rich in shallot confer protection against cancer and finally introduce new agents with pharmacological activities in shallot as a potential candidate in cancer therapy.
Famil Samavati, S., Mohammadi-Motlagh, H. & Mostafaie, A. (2014). A Highly Pure Sub-Fraction of Shallot Extract With Potent in vitro Anti-Angiogenic Activity. International Journal of Complementary and Integrative Health, 3(4), 237-245.
MLA
Famil Samavati, S., Mohammadi-Motlagh, H., & Mostafaie, A. "A Highly Pure Sub-Fraction of Shallot Extract With Potent in vitro Anti-Angiogenic Activity", International Journal of Complementary and Integrative Health, 3, 4, 2014, 237-245.
HARVARD
Famil Samavati S., Mohammadi-Motlagh H., Mostafaie A. (2014). 'A Highly Pure Sub-Fraction of Shallot Extract With Potent in vitro Anti-Angiogenic Activity', International Journal of Complementary and Integrative Health, 3(4), pp. 237-245.
CHICAGO
S. Famil Samavati, H. Mohammadi-Motlagh & A. Mostafaie, "A Highly Pure Sub-Fraction of Shallot Extract With Potent in vitro Anti-Angiogenic Activity," International Journal of Complementary and Integrative Health, 3 4 (2014): 237-245,
VANCOUVER
Famil Samavati S., Mohammadi-Motlagh H., Mostafaie A. A Highly Pure Sub-Fraction of Shallot Extract With Potent in vitro Anti-Angiogenic Activity. Int J Complement Integr Health. 2014;3(4):237-245.