Cytotoxic Activity of Bioactive Compound 1, 2- Benzene Dicarboxylic Acid, Mono 2- Ethylhexyl Ester Extracted from a Marine Derived Streptomyces sp. VITSJK8

Document Type : Review Article

Authors
Kannabiran Krishnan
Abirami Mani
Subashini Jasmine
School of Biosciences and Technology, VIT University, Tamilnadu, India
Abstract
Marine Streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in
pharmaceutical industry as effective drugs against life threatening diseases. The cytotoxic activity of the pure
compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (DMEHE) from marine derived
actinomycete Streptomyces sp. VITSJK8 was investigated against mouse embryonic fibroblast (NIH 3T3) and
human keratinocyte (HaCaT) normal cell lines, human hepatocellular liver carcinoma (HepG 2) and human
breast adenocarcinoma (MCF-7) cell lines by using MTT assay. The compound DMEHE exhibited IC 50 values
of 42, 100, 250 and 500 µg/ ml against HepG2, MCF-7, HaCaT and NIH 3T3 cell lines, respectively. The effect
of DMEHE on the growth of cancer cell lines was expressed as the % of viability. Cell viability was recorded as
67.7%, 78.14%, 82.23% and 96. 11% in HepG2, MCF-7, HaCaT and NIH 3T3 cells, respectively. The results of
the study conclude that the bioactive compound isolated from the potential isolate Streptomyces sp. VITSJK8
exhibited cytotoxic activity against HepG2 and MCF- 7 cancer cell lines and low toxicity against normal HaCaT
and NIH 3T3 cell lines.
Keywords
Streptomyces sp. VITSJK8
1
2- benzene dicarboxylic acid
mono 2- ethylhexyl ester
cytotoxicity
MTT assay