The Effect of Adiponectin on Osteonectin Gene Expression by Oxidized Low Density Lipoprotein-Treated Vascular Smooth Muscle Cells

Document Type : Original Article

Authors
Sara Niknam 1
Keihan Ghatreh-Samani 1
Effat Farrokhi 2
1 Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract
Osteonectin is a bone- associated protein involved in vascular calcification. Adiponectin may protect against
cardiovascular disease but possible effects on vascular calcification have been poorly studied. The aim of this
study was to investigate the modulatory effect of adiponectin on oxidized low density lipoprotein (oxLDL)-
induced expression of osteonectin in human aorta vascular smooth muscle cells (HA/VSMCs). HA/VSMCs were
cultured in F12K media and then treated with oxLDL (100 µg/mL) in the presence or absence of adoponectin (5
µg/mL) for 24 and 48 hours. mRNA expression and protein level of osteonectin were determined by quantitative
real-time PCR and western blot analysis, respectively. After exposure to oxLDL, osteonectin expression
increased 1.62 ± 0.23- and 6.62 ± 0.48-fold after 24 and 48 hours respectively compared to the control.
Adiponectin increased oxLDL- induced osteonectin expression in a time-dependent manner after 24 and 48
hours (3.24 ± 0.39- and 24.93 ± 2.15-fold, respectively). Western blotting confirmed that osteonectin protein was
upregulated by adiponectin.Our data suggest that OxLDL might cause the increase of osteonectin expression
both at mRNA and protein level. This upregulation is intensified by adiponectin.
Keywords
Vascular smooth muscle cells
oxidized low density lipoprotein
adiponectin
osteonectin