Document Type : Original Article
Authors
1
Department of Chemistry, Annamalai University, Annamalai Nagar-608002, Tamilnadu, India
2
Department of Clinical Neurosciences, University of Calgary, Calgary, Alberta T2N 4N1, Canada
3
Chemistry section, FEAT, Annamalai University, Annamalai nagar-608002, Tamilnadu, India
4
Drug Theoretics and Cheminformatics Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Jadavpur, Kolkata, 700032, India
5
Manchester Institute of Biotechnology, University of Manchester, Manchester M1 7DN, Great Britain
Abstract
The current study highlights the in vitro antioxidant and antitumor activity of the previously-synthesized
hydrazone derivatives against various free radicals and human cancer cell lines, respectively. The anticancer
efficacies of the compound were tested by measuring cytotoxicity in cancer cell lines HeLa, A549, and non-
cancerous NL20 cells. Compounds possessing electron-donor methoxy and methyl substitutions at the para
position of the phenyl ring moiety showed a concentration dependent free radical scavenging effects. The free
radical-scavenging potential of synthetic compounds 11 and 14 may have significant impact on the prevention of
free radical-induced oxidative stress and carcinogenesis. The results from cytotoxicity and cell migration assay
showed that the substitution of electron-withdrawing fluoro, chloro and bromo functional groups induced a
significant (P< 0.001) loss of cell viability and inhibited the invasive potential of the human cancer cells.
Additionally, these compounds showed significantly (P< 0.05) a less toxicity toward non-cancerous NL20 cells.
Docking studies revealed interactions of compound 10 with p38α MAP kinase, which may be responsible of its
anti-invasive and anti-proliferative effects.
Keywords