Expression Pattern of Neuronal Markers in PB-MSCs Treated by Growth Factors Noggin, bFGF and EGF

Document Type : Original Article

Authors
Zahra Fazeli 1
Sayyed Mohammad Hossein Ghaderian 1 , 2
Masoumeh Rajabibazl 3
Siamak Salami 3
Nader Vazifeh Shiran 4
Mir Davood Omrani 1 , 2
1 Department of Medical Genetics, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Shahid Labbafi Nejad Educational Hospital, Tehran, Iran
3 Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Department of Hematology, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Mesenchymal stem cells (MSCs) have the ability to differentiate into neuronal like cells under appropriate
culture condition. In this study, we investigated whether MSCs derived from human peripheral blood (PB-
MSCs) can differentiate into neuronal like cells by synergic effect of the growth factors EGF, bFGF and Noggin.
For this purpose, the expression of five neuronal markers (Nestin, β III tubulin, NFM, MAP2 and NSE) were
evaluated in treated PB-MSCs by SYBR Green Real time PCR. The expression analysis showed a higher
expression of β- tubulin and NFM in treated BP-MSCs compared with untreated PB-MSCs as a control group.
The expression of Nestin was also diminished in PB-MSCs treated with Noggin. This study suggested that the
treatment of PB- MSCs with Noggin alongside with bFGF and EGF might differentiate these cells into neuronal
lineage cells. The obtained results could be further developed for useful applications in regenerative medicine.
Keywords
Mesenchymal stem cells
differentiation
neuronal markers
Noggin