Dual Effects of Resveratrol on the Expression and Secretion ofAngiogenic Factors

Angiogenesis is an essential process in the growth, development ,and transition of
tumors from dormancy to proliferating state. Resveratrol (RSV), as a natural
polyphenolic compound, is claimed to be effective in regulating angiogenesis. This study
aimed to evaluate the impact of RSV onthe angiogenesis process in HUVECs (human
umbilical vein endothelial cells) alone and co
-cultured with Jurkat cells. The effects of
RSV on HUVECs and Jurkat cell viability and apoptosis were measured by MTT and
Annexin
-V/PI methods. HUVECs were co
-cultured with pre
-treated Jurkat cells and
incubated for 24 h, 48 h and 72 h. The angiogenesis process in HUVECs and Jurkat cells
alone and in co
-culture models was investigated by analyzing the expression of VEGF,
VEGFR
-
2
,and Interleukin
-
8 (IL
-8) employing qPCR and ELISA. RSV at low
concentration (40 µM) had no significant effects on apoptosis rate of HUVECs, but
higher concentrations (80
-160 µM) increased apoptosis in co
-culture method and
HUVECs alone. RSV significantly reduced VEGFR2 and IL
-8 gene expression also, IL
-
8 protein concentration in HUVECs, but the effects of this drug in the HUVECs
-Jurkats
co
-culture were different. Expression of VEGF in Jurkat cells increased following
treatment with RSV. RSV had direct anti
-angiogenic effects on HUVECs. Unexpectedly
its indirect effects were not significant on HUVECs
-Jurkats co
-culture. Results of our
study showed, RSV may be effective in anti
-angiogenesis therapy, but in some
situations, it may induce angiogenesis. So, appropriate concentrations should achieve to
minimize the unpredicted effects of RSV