Increased Expression of ITGB 3 in CLL Patient leukemia Cells byExposure to Cold Physical Plasma and Plasma-treated Medium

Document Type : Original Article

Authors
Monireh Golpour 1 , 2
Farshad Sohbatzadeh 3
Mina Alimohammadi 4
Zahra Yazdani 2 , 5
Sadegh Fattahi 6
Ehsan Zaboli 7
Alireza Rafiei 2 , 5
Sander Bekeschus 8
1 Cancer Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
2 Molecular and Cell Biology Research Center (MCBRC), Hemoglobinopathy Institute, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
3 Department of Atomic and Molecular Physics, Faculty of Science, University of Mazandaran, Babolsar, Iran.
4 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5 Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
6 North Research Center of Pasteur Institute, Amol, Iran.
7 Department of Internal Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
8 ZIK Plasmatis, Leibniz Institute for Plasma Science and Technology (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
10.22088/IJMCM.BUMS.13.3.248
Abstract
Chronic lymphocytic leukemia (CLL) is the most prevalent hematological cancer, with various medical
interventions. In the recent decade, cold physical plasma has become an interesting agent for future cancer
therapy. The goal of this study was to see whether cold physical plasma or cold physical plasma-treated liquid
(PTL) affected integrin beta 3 (ITGB3) expression, which is hypothesized to mediate an interaction between
cancer stem cells and the bone marrow microenvironment, in CLL patients' blood cells. The metabolic activity,
cell death pattern, lipid oxidation and ITGB3 gene expression of these treatments was evaluated. Both direct
cold physical plasma and PTL exposure enhanced lipid peroxidation in cells of CLL patients, but to a lesser
extent in healthy participants. Furthermore, following 48h of cold physical plasma or PTL exposure, the
metabolic activity of leukocytes was preferentially reduced in CLL patient leukocytes. In addition, cold
physical plasma and PTL treatment elevated ITGB3 mRNA expression in CLL patients' leukocytes compared
to untreated and healthy controls. Collectively, our study suggests selective effects of direct cold physical
plasma and PTL exposure on blood leukocytes from leukemia patients, but further and more detailed studies
are needed to provide additional rationales for such treatment options as future therapy.
Keywords
CAP
cold atmospheric pressure plasma
chronic lymphocytic leukemia
gas plasma technology
NK cells
reactive oxygen species
ROS