Down Expression of Zyxin is Associated with Down Expression of p53 inColorectal Cancer

Document Type : Original Article

Authors
Hossein Mohammadi 1
Ebrahim Shakiba 2
Rezvan Rostampour 3
Kiana Bahremand 4
Mohammad Taghi Goodarzi 5
Homayoon Bashiri 6
Khadijeh Najafi Ghobadi 7
Soheila Asadi 1 , 8
1 Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2  Department of Clinical Biochemistry, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 Clinical Research Development Center, Imam Reza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran.
4 Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
5 Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran.
6 Department of Internal Medicine, Infectious Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.
7 Department of Biostatistics, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran.
8 Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
10.22088/IJMCM.BUMS.14.1.462
Abstract
Colorectal cancer (CRC), which is among the most prevalent cancers worldwide, is caused by
environmental and genetic factors. It has been shown that the p53 gene is associated with CRC
pathogenesis; moreover, Zyxin (ZYX) may play a role in p53 level and activity. Therefore, the
present research aimed to investigate the levels of P53 and ZYX genes and proteins in CRC tumor
samples. Cancerous tissues (n=31) and matched non-cancerous tissues (n=31) were randomly
obtained from 31 patients with CRC. Total RNA was extracted using RNXplus, and gene
expressions were assessed by Real-time PCR. Furthermore, the Western blot technique was used to
investigate the expression of ZYX and P53 proteins. The obtained results revealed that the
expression of ZYX and p53 genes in cancerous tissues showed no significant difference compared to
matched non-cancerous tissues. On the other hand, measuring protein expression using the Western
blotting technique indicated that the ZYX (P=0.0081) and p53 (P=0.0065) expression in tumor
tissues significantly decreased compared to those in matched non-cancerous tissues. Correlation
analysis indicated a significant correlation between ZYX and P53 proteins (r=0.746, P=0.013).
Based on our findings, ZYX might have a suppressive function in CRC and is associated with P53.

Keywords
Colorectal neoplasms
Tumor suppressor protein p53
Zyxin