Elucidating the Role of LINC01980 in Laryngeal Cancer: FromExpression Profiling to Regulatory Network Prediction

Document Type : Original Article

Authors
Masoumeh Razipour 1
Zeinab Jamali 1
Elham Ghadami 2
Saeed Sohrabpour 3
Leyla Sahebi 4
Abbas Shakoori 1 , 5
1 Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
2  Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
3 Otorhinolaryngology Research Center, AmirAlam Hospital, Tehran University of Medical Sciences, Tehran, Iran.
4 Family Health Research Institute, Maternal-Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran.
5 Department of Medical Genetics, Cancer Institute of Iran, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran.
10.22088/IJMCM.BUMS.14.1.517
Abstract
Laryngeal squamous cell carcinoma (LSCC) remains a significant global health challenge despite
advances in treatment. This study investigated the involvement of LINC01980, a long non-coding
RNA, in LSCC pathogenesis. We conducted an integrative analysis of transcriptomic data sourced
from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases to
identify genes that are differentially expressed in LSCC. LINC01980 expression was evaluated in 32
matched pairs of advanced-stage LSCC and adjacent normal tissues using quantitative real-time PCR
(qRT-PCR). The potential competing endogenous RNA (ceRNA) network involving LINC01980 was
explored through bioinformatics analyses. Findings revealed significant upregulation of LINC01980
in LSCC tissues compared to normal adjacent tissues (p < 0.0001), with elevated expression in
78.125% of tumor samples. Receiver Operating Characteristic (ROC) curve analysis demonstrated
LINC01980's potential as a diagnostic biomarker (AUC = 0.7666, p = 0.0002). While no significant
correlations were found between LINC01980 expression and clinicopathological features,
bioinformatics analyses identified potential LINC01980/ miRNA/ mRNA regulatory network
involving hsa-let-7e-5p and hub gene MMP9. This study provides insights into LINC01980's role in
LSCC and suggests its potential as a diagnostic biomarker and therapeutic target. Further research is
warranted to elucidate the precise molecular mechanisms of LINC01980 in LSCC progression.

Keywords
Laryngeal Squamous Cell Carcinoma
Long Non-Coding RNA
competing endogenousRNA
LINC01980
biomarker