Vitamin E incorporated in Carboxymethyl Cellulose-Gelatin HydrogelIncreases Wound Healing

Document Type : Original Article

Authors
Seyed Reza Mousavi 1
Mojtaba Rashidi 1 , 2
Azam Khedri 1
Maryam Kouchak 3
Majid Salehi 4
Ghorban Mohammadzadeh 1 , 2
1 Department of Clinical Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
2 Hyperlipidemia Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
3 Department of Pharmaceutics, Faculty of Pharmacy, Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
4 Regenerative Medicine Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
‎10.22088/IJMCM.BUMS.14.3.808
Abstract
Improving wound care products to facilitate effective skin repair is very important.
Hydrogels are promising polymer-based dressings that enhance wound healing. Vitamin E
can improve skin injuries by increasing antioxidant capacity. This study aimed to construct
an innovative hydrogel from carboxymethyl cellulose (CMC) and gelatin (Gel) containing
vitamin E to enhance wound healing.
Five unique hydrogel formulations were constructed by combination of CMC-Gel and 25,
50, 100, and 200 µL/mL of Vitamin E. Structural characteristics of hydrogels were assessed
using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy
(FTIR). Biochemical properties of hydrogels, including swelling, weight loss, pH values,
and blood compatibility, were evaluated by specific methods. The cytotoxicity effects of
hydrogels on the NIH-3T3 fibroblasts were determined by MTT assay. The therapeutic
potential of the hydrogels was investigated using a full-thickness wound model in Wistar
rats. The constructed CMC-Gel-Vit E hydrogel had a porous structure characterized by
interconnected voids measuring 73.15±9.61 µm, which is favorable for promoting cell
migration. The cytotoxicity results showed no toxicity effects of hydrogels with and without
VitE 100 µL/mL, also a survival rate of over 120% in cells after 72 h. In vivo data showed
the CMC-Gel-Vit E hydrogel (91.36±8.23%) significantly increased the percentage of
wound closure and re-epithelialization compared to the control group (68.31±13.59%).
The results highlight the considerable potential of the CMC-Gel-VitE hydrogel as a viable
option for skin regeneration and wound healing. This hydrogel exhibits substantial promise
for use in clinical and therapeutic interventions.
Keywords: Skin Regeneration, Hydrogel dressing, NIH 3T3 Cells, Tocopherols,
Biopolymer
Keywords
Skin Regeneration
Hydrogel dressing
NIH 3T3 Tocopherols
Biopolymer