Document Type : Original Article
Authors
1
Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
2
Department of Nutritional Health Research Center, School of Health and Nutrition, Lorestan University of Medical Sciences, Khorramabad, Iran.
3
Department of Physiology and Pharmacology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
4
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
5
Nutritional Health Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
Abstract
Gold nanoparticles (AuNPs) are a suitable choice in the biomedical field due to their
physical properties. The presence of gold nanoparticles, acting alongside
mesenchymal stem cells (MSCs) through paracrine interactions, accelerates wound
closure, enhances angiogenesis, favorably regulates extracellular matrix remodeling,
and encourages skin regeneration with typical construction and function. In this
study, we hypothesized that AuNPs Nano scaffolds would enhance the proliferation
and specialization potential of human adipose-derived mesenchymal stem cells
(hADSC in the wound area during in vitro, In vivo studies. To evaluate the
nanoparticles; transmission electron microscopy was used to detect AuNPs and
dynamic light scattering assay was used to estimate their size. Immunofluorescence
staining, MTT assay, and semi-quantitative histological analysis were performed.
Our results show that AuNPs at non-toxic concentrations (6 ppm) improve collagen
content deposition and micro vessels expression induces cell migration and
therapeutic efficacy in wound healing by hADSC at 14 and 28 days after surgery. As
a result, AuNPs promote differentiation through a paracrine signaling pathway.
Therefore, hADSC cultured with AuNPs can be used as a reagent to enhance skin
tissue formation.
Keywords: Dynamic Light Scattering, Paracrine Communication, Metal
Nanoparticles, Collagen, Fluorescent Transmission, Cell Proliferation, Angiogenesis
Keywords