Vitexin Induces Apoptosis in MCF-7 Breast Cancer Cells through theRegulation of Specific miRNAs Expression

Document Type : Original Article

Authors
Reza Najafipour 1
Abdol Mabood Momeni 2
Yousef Mirmazloomi 3
Sahar Moghbelinejad 4
1 Genetics Research Center, University of Social Welfare and Rehabilitation Science, Tehran, Iran.
2 Khatam Pathobiology and Genetics lab, Qazvin, Iran.
3 School of Medicine, Qazvin University of Medical Sciences, Shahid Bahonar Boulevard, Qazvin, Iran.
4 Research Institute for Prevention of Non-Communicable Diseases, Cellular and Molecular Research Centre, Qazvin University of Medical Sciences, Shahid Bahonar Boulevard, Qazvin, Iran.
10.22088/IJMCM.BUMS.11.3.197
Abstract
In this research, we investigated microRNAs (miRNAs) expression profile in MCF-7 breast
cancer cell line which treated with 150 μM vitexin. Profiling of miRNAs expression was
performed using TaqMan MiRNA Array. Apoptosis was analyzed by flow cytometry and the
expression of some genes involved in "anti-proliferative" signaling pathways were evaluated by
western blotting and real time PCR methods. Twenty microRNAs were differentially expressed
in vitexin treated cells compared to the control. Among them, let-7- b, c were up regulated while
miRNA-17-5p was down regulated with highest score. Also, we detected the expression changes
of mentioned miRNAs target genes as well as genes involved in caspase apoptosis pathways. Our
results provide the first evidence that vitexin can effect miRNA expression in MCF-7 cells. Also
based on our finding, vitexin can be an attractive miRNA mediated chemo preventive and
therapeutic agent in breast cancer.